TABLE 2.
Spore formation, recombination, and spore viabilitya
| Assay |
WT
|
grr1Δ
|
||
|---|---|---|---|---|
| Sp | Sp + glucose | Sp | Sp + glucose | |
| Spore formation | 52 ± 3 | 25 ± 5 | 44 ± 3 | 4.3 ± 0.9 |
| Dyads, total asci (%) | 34 ± 2 | 49 ± 7 | 64 ± 3 | 93 ± 4 |
| LEU2-MAT recomb. (cM) | 33 ± 2 | 30 ± 2 | 38 ± 1 | 34 ± 3 |
| Spore viability (%) | 93 ± 1 | 96 ± 2 | 71 ± 3 | 44 ± 8 |
a
Mid-log-phase cultures were transferred to Sp or Sp plus glucose medium, incubated for 72 h, and then assayed for spore formation, the fraction of dyads among total asci, recombination (recomb.) in the LEU2-MAT interval, and spore viability. Recombination was assayed in SH2898 (grr1Δ/grr1Δ) or SH2896 (grr1Δ/GRR1); all other experiments were assayed in SH1752 (grr1Δ/grr1Δ) or SH1970 (WT). Values are means ± standard errors. WT, wild type.