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Endogenous CUL3, KEAP1, and NRF2 form a complex. 293T cells were treated with MG132 or DMSO (−) for 4 h prior to lysis. Cell lysates were immunoprecipitated (IP) with antibodies to CUL3 (α-CUL3), KEAP1 (α-KEAP1), or NRF2 (α-NRF2). Washed immunocomplexes were resolved by SDS-PAGE, followed by immunoblotting (IB) with the indicated antibodies. IgG (H), immunoglobulin G (heavy chain).
