FIG. 9.

RsmA and RsmE proteins stabilize RsmY and RsmZ RNAs. (A) RsmY and RsmZ transcript decay in the wild-type strain P. fluorescens CHA0 (wt) and in the rsmA rsmE double mutant CHA1009 was determined by Northern blotting after blocking transcription with rifampin. The amount of RNA loaded was 0.5 μg for the wild type and 3 μg for the rsmA rsmE double mutant. As both rsmY and rsmZ are poorly expressed in the rsmA rsmE background, the stability of RsmY and RsmZ was studied in CHA1009 expressing rsmY or rsmZ from the tac promoter of pME6918 (rsmY) or pME6359 (rsmZ). (B) Densitometric analysis of RsmY stability in CHA0 (solid circles) and CHA1009 (open circles). (C) Densitometric analysis of RsmZ stability in CHA0 (solid circles) and CHA1009 (open circles).