TABLE 2.
Prediction of the primary structure of the SprE forms on the basis of N-terminal amino acid sequence and MALDI-TOF mass spectroscopy analysis
| Strain | SprE form (fraction) | N-terminal sequence | Experimentally determined molecular mass (Da) | Theoretical molecular mass of the inferred fragment (Da) | Predicted structure |
|---|---|---|---|---|---|
| TX4002 | 1L-SprE (F′) | LLDPE | 25,216 | 25,215.7 | Leu1-Arg230 |
| TX5264 | 1L-SprE (F) | LLDPE | 25,826 | 25,525.5 | Leu1-Ala237 |
| 1L-SprE (G1) | LLPDE | 24,820 | 24,817.3 | Leu1-Glu227 | |
| −1S-SprE (G2) | SLLDP | 24,902 | 24,904.4 | Ser−1-Glu227 |