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Growth (OD600, solid lines) and release of cytoplasmic proteins (dashed lines) by L. lactis PH3960 (Δalr) and L. lactis GB3960 (Δalr acmAΔ1). The two strains were grown in GM17 medium with 2 mM d-Ala until exponential phase, after which d-Ala was removed from the growth medium by centrifugation and cells were resuspended in fresh GM17 broth without d-Ala.
