TABLE 2.
Effect of glycine on lysis of L. lactis NZ3900 (control) and L. lactis PH3960 (Δalr) in the presence or absence of d-Alaa
| Strain | % Glycine added | % of maximum OD600
|
|
|---|---|---|---|
| With D-Ala | Without D-Ala | ||
| NZ3900 | 0 | 98 | 94 |
| NZ3900 | 1 | 94 | 97 |
| NZ3900 | 2 | 89 | 84 |
| PH3960 (Δalr) | 0 | 99 | 30 |
| PH3960 (Δalr) | 1 | 83 | 5.7 |
| PH3960 (Δalr) | 2 | 60 | 5.9 |
a
Strains were grown until an OD600 of 0.5 was reached, after which the cultures were split in two, centrifuged, washed once with an equal volume of fresh medium, and resuspended in fresh medium. d-Ala (2 mM) and/or glycine (1 or 2%) were added and growth was followed during 22 h.