Table. Molecular analysis of influenza A subtype H5 viruses emerging in November 2016 compared with previously isolated H5 viruses*.
| Virusesa | HA clade classification | HA sequence (aa) | HA deletion |
NA stalk deletion | NS1 | PB2 sequence at aa | Expression of PB1-F2 protein | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cleavage site | Receptor binding sites | Deletion of aa 80–84 | Aa residue at | |||||||||||||||
| 335-348b | 158 | 193 | 222 | 224 | 226 | 227 | 228 | 318 | 133 | 49-68 | 92 | C-term | 627 | 701 | ||||
| EM/Korea/W541/16 | 2.3.4.4 | RERRR_KR/G | N | N | Q | N | Q | Q | G | T | YES | YES | YES | E | ESEV | E | D | YES |
| Yunnan/China/0127/15c | 2.3.4.4 | RERRR_KR/G | N | N | Q | N | Q | R | G | T | YES | YES | NO | D | KPEV | K | D | YES |
| Ck/Sichuan/NCJPL1/2014 | 2.3.4.4 | RERRR_KR/G | N | N | Q | N | Q | R | G | T | NO | NO | YES | E | ESEV | E | D | YES |
| MDk/Korea/W452/14 | 2.3.4.4 | RERRR_KR/G | N | N | Q | N | Q | R | G | T | NO | NO | NO | D | ESEV | E | D | YES |
| BDk/Korea/Gochang1/14 | 2.3.4.4 | RERRR_KR/G | N | N | Q | N | Q | R | G | T | NO | NO | NO | D | ESEV | E | D | YES |
| Em/Korea/W149/06 | 2.2 | GERRRKKR/G | N | K | K | N | Q | S | G | T | NO | YES | YES | D | ESKV | K | D | YES |
| MDk/Korea/W401/11 | 2.3.2 | RERRR_KR/G | D | R | K | N | Q | S | G | T | NO | YES | YES | D | ESEV | E | D | YES |
| Egypt/MOH/7271/14 c | 2.2.1.2 | GERRRKKR/G | N | R | K | N | Q | S | G | T | YES | YES | YES | D | ESEV | K | D | YES |
Aa: amino acid; BDk: Breeder duck; C-term: 4 amino acid sequence at the C-terminal end; Em: environment; HA: haemagglutinin; HPAI: highly pathogenic avian influenza; MDk: mallard duck; RBS: receptor binding site; MOH: Ministry of health; NA: neuraminidase; NS: nonstructural protein; PB: polymerase basic protein.
The accession numbers of each virus are followed: Yunnan/China/0127/15 : KT245143~KT245150, Ck/Sichuan/NCJPL1/2014: PB2-KM251533, PB1- KM251523, PA-KM251513, HA-KM251463, NP-KM251493, NA-KM251486, M-KM251473, and NS-KM251503, MDk/Korea/W452/14 : KJ746108~KJ746115, BDk/Korea/Gochang1/14 : KJ413831~KJ413838, EM/Korea/W149/06 : EU233731~EU233738, MDk/Korea/W401/11 : JN202558~JN202572, Egypt/MOH/7271/14 : KP702162~KP702169, and EM/Korea/W541/16 : KY272997~KY273025
a The isolates in boldface are the 2016 Korean HPAI H5N6 virus examined in this study.
b H3 numbering.
c Human isolates.