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Schematic summarizing the data described in the text. With the exception of V230D in the connector, all HAMP mutations that abolished FAD binding affected residues (white circle) in the AS-2 region. The signaling domain of Aer controls the direction of rotation of the flagellar motor by modulating autophosphorylation of CheA and phosphorylation of CheY (see Fig. 1 in reference 31).
