TABLE 2.
Behavioral assays of Aer mutants exhibiting super-swarming or an inverse response
| Plasmida | Protein | Swarm sizeb,c (mm) | Band distancec (mm) | Response time (s)c,d
|
Aer expressione | |
|---|---|---|---|---|---|---|
| O2 increase | O2 decrease | |||||
| pGH1 | Wild-type Aer | 20.0 ± 0.0 | 18.5 ± 2.1 | 143.0 ± 23.4 | 25.7 ± 3.6 | +++ |
| pQH68 | AerK221E | 39.6 ± 8.1 | 11.5 ± 0.7 | 131.7 ± 15.1 | 25.2 ± 4.2 | +++ |
| pQH69 | AerV222A | 31.5 ± 1.5 | 16.3 ± 1.8 | 93.7 ± 31.5 | 30.8 ± 4.4 | +++ |
| pQH70 | AerA223V | 39.6 ± 7.4 | 13.0 ± 4.2 | 102.7 ± 8.7 | 30.3 ± 0.6 | +++ |
| pQH58 | AerE226V | 45.5 ± 2.5 | NDf | 59.0 ± 7.4 | 24.8 ± 2.3 | +++ |
| pQH74 | AerV230A | 31.3 ± 6.7 | 21.5 ± 2.1 | 70.7 ± 13.0 | 11.2 ± 2.0 | + to ++ |
| pQH76 | AerD237E | 40.0 ± 8.5 | 43.0 ± 11.3 | 26.2 ± 8.3 | 18.4 ± 3.8 | ++ |
| pQH81 | AerG240E | 39.8 ± 6.0 | 13.5 ± 2.1 | 103.0 ± 10.4 | 22.7 ± 3.1 | +++ |
| pQH84 | AerG247R | 34.5 ± 10.6 | 16.5 ± 2.1 | 114.0 ± 14.9 | 16.0 ± 3.5 | ++ to +++ |
| pQH85 | AerQ248L | 40.8 ± 7.4 | 12.5 ± 3.5 | 84.5 ± 0.7 | 18.0 ± 4.0 | +++ |
| pQH93 | AerS261L | 31.5 ± 9.2 | 13.8 ± 1.8 | 150.0 ± 0.0 | 30.0 ± 2.0 | +++ |
| pQH95 | AerV264A | 30.4 ± 1.2 | 15.5 ± 3.5 | 58.2 ± 14.8 | 21.0 ± 3.4 | +++ |
| pQH96 | AerV264M | 12.2 ± 1.6 | No band | −(135.3 ± 9.0) | −(17.0 ± 1.8) | +++ |
| pQH98 | AerS266C | 43.1 ± 4.4 | 10.0 ± 0.0 | 92.5 ± 18.2 | 26.3 ± 4.4 | +++ |
| pQH100 | AerE272G | 41.2 ± 1.6 | 17.0 ± 0.0 | 89.3 ± 10.2 | 29.8 ± 6.3 | +++ |
a
All plasmids were derived from the pTrc99A expression vector. Cells expressing pQH96 had an inverse aerotaxis response. Cells expressing all other plasmids had a superswarming phenotype.
b
Swarm sizes were normalized to 20 mm for pGH1.
c
The data represent means ± standard deviations of results from more than two independent experiments.
d
Negative values represent inverse responses.
e
+, ++, +++, graduated levels of proteins detected in the whole-cell extracts of strains without induction.
f
Not determined.