TABLE 4.
lsr-lacZ expression in E. coli mutants insensitive to catabolite repression grown in LB medium with G3P or glycerol
| Genotype | β-Galactosidase activity (u)a
|
Fold repression with:
|
|||
|---|---|---|---|---|---|
| LB medium | LB medium + G3P | LB medium + glycerol | G3P | Glycerol | |
| WT | 1,071 ± 66 | 226 ± 2 | 50 ± 1 | 5 | 21 |
| cya crp* | 1,094 ± 46 | 886 ± 12 | 137 ± 8 | 1 | 8 |
a
The β-galactosidase activities of the lsr-lacZ fusion were measured in strains KX1123 (WT) and KX1468 (Δcya crp*) following 5 h of growth in LB medium or LB medium containing 0.4% G3P or 0.4% glycerol.