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. 2017 Apr 3;12:2645–2656. doi: 10.2147/IJN.S132670

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© 2017 Ge et al. This work is published and licensed by Dove Medical Press Limited

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Sensitivity test results of the mRT-LAMP-CIRN assay for detecting influenza A/H1N1pdm09, A/H3 and influenza B.

Notes: Ten-fold serial dilutions of A/H1N1pdm09 (A), A/H3 (B) and influenza B (C) RNA transcripts (ranging from 10⁴ to 10⁰ RNA copies) were detected by the mRT-LAMP-CIRN assay. The multiplex RT-LAMP amplification reactions for A/H1N1pdm09 (D), A/H3 (E) and influenza B (F) RNA transcripts were also real-time monitored by a turbidimeter and the corresponding curves of concentrations of templates were marked in the figure.

Abbreviations: mRT-LAMP-CIRN, multiplex reverse-transcription loop-mediated isothermal amplification coupled with cascade invasive reaction using nanoparticles as a sensor; DW, distilled water.