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. 2017 Apr 3;12:2645–2656. doi: 10.2147/IJN.S132670

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© 2017 Ge et al. This work is published and licensed by Dove Medical Press Limited

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Specificity test results of the mRT-LAMP-CIRN assay for detection of influenza viruses.

Notes: The analytical specificity of the mRT-LAMP-CIRN assay was assessed by testing the RNA extracts from influenza A/H1N1pdm09, A/H3N2 and influenza B, and from various control viruses separately. RNA extracts from the three influenza virus strains were also tested in different combinations in a single reaction. (A) The cascade invasive reactions were performed with 09H1-Up, 09H1-Dp and Hp. (B) The cascade invasive reactions were performed with H3-Up, H3-Dp and Hp. (C) The cascade invasive reactions were performed with FluB-Up, FluB-Dp and Hp. 1: no-template control; 2: A/H1N1pdm09; 3: A/H3N2; 4: influenza B; 5: A/H1N1pdm09 and A/H3N2; 6: A/H1N1pdm09 and influenza B; 7: A/H3N2 and influenza B; 8: A/H1N1pdm09, A/H3N2 and influenza B; 9: A/H1N1; 10: A/H5N1; 11: A/H7N9; 12: A/H9N2; 13: respiratory syncytial viruses type A; 14: respiratory syncytial viruses type B; 15: parainfluenza viruses types 1; 16: parainfluenza viruses types 2.

Abbreviations: mRT-LAMP-CIRN, multiplex reverse-transcription loop-mediated isothermal amplification coupled with cascade invasive reaction using nanoparticles as a sensor; Up, upstream probe; Dp, downstream probe; Hp, hairpin probe.