Table 1.

Primers used for multiplex RT-LAMP assay

Target	Name	Type	Sequence (5′-3′)	Length (nt)
A/H1N1pdm09, (HA gene)	09H1-F3	Forward outer	CCGGGAGACAAAATAACATTC	21
	09H1-B3	Reverse outer	GTATATTCTGAAATGGGAGGC	21
	09H1-FIB	Forward inner	CAGATCCAGCATTTCTTTCCATTGGAAG CAACTGGAAATCTAGTG(F1C+F2)	45
	09H1-BIP	Reverse inner	TATCATTTCAGATACACCAGTCCACTGG TGTTTATAGCACCCTTG(B1C+B2)	45
	09H1-LF	Forward loop	CGAATGCATATCTCGGYAC	19
	09H1-LB	Reverse loop	ATACAACTTGTCARACACC	19
A/H3, (M1 gene)	H3-F3	Forward outer	AGGATGGGGGCTGTAACC	18
	H3-B3	Reverse outer	CCAGCCATTTGCTCCATAGC	20
	H3-FIB	Forward inner	TGAGACCTGTGCTGGGAGTCAGGTG GCMTTTGGCCTGGTA	40
	H3-BIP	Reverse inner	TAGGCARATGGTGGCAACAACCTGT AGTGCTGGCCARAACC	41
	H3-LF	Forward loop	AATCTGYTCACATGTTGCACA	21
	H3-LB	Reverse loop	CATTAATAARACATGAGAACAGAAT	25
Influenza B, (NS1 gene)	FluB-F3	Forward outer	AGGGACATGAACAACAAAGA	20
	FluB-B3	Reverse outer	CAAGTTTAGCAACAAGCCT	19
	FluB-FIB	Forward inner	TMARGGACAATACATTACGCATATC GATAAAGGAGGAAGTAAACACTCA	49
	FluB-BIP	Reverse inner	TAAAYGGAACATTCCTCAAACACCACTC TGGTCATAGGCATTC	43
	FluB-LF	Forward loop	TYAAACGGAACTTCCCTTCTTTC	23
	FluB-LB	Reverse loop	GGATACAAGTCCTTATYAACTCTGC	25

Notes: The polymorphic sites of the primers are underlined; Y represents degenerate C or T; R represents degenerate A or G; M represents degenerate A or C.

Abbreviations: RT-LAMP, reverse-transcription loop-mediated isothermal amplification; HA, hemagglutinin; M1, matrix protein 1; NS1, nonstructural protein 1; nt, nucleotide.