Table 3.
Clinical performance of mRT-LAMP-CIRN compared with real-time RT-PCR for detecting influenza A/H1N1pdm09, A/H3 and influenza B
| mRT-LAMP-CIRN | Real-time RT-PCR
|
Performance characteristics
|
||
|---|---|---|---|---|
| Positive | Negative | Sensitivity, % | Specificity, % | |
| A/H1N1pdm09 | ||||
| Positive | 34 | 0 | 97.1 | 100 |
| Negative | 1 | 59 | ||
| A/H3 | ||||
| Positive | 14 | 0 | 100 | 100 |
| Negative | 0 | 80 | ||
| Influenza B | ||||
| Positive | 9 | 0 | 100 | 100 |
| Negative | 0 | 85 | ||
| Combined A/H1N1pdm09, A/H3 and FluB | ||||
| Positive | 57 | 0 | 98.3 | 100 |
| Negative | 1 | 36 | ||
Abbreviations: mRT-LAMP-CIRN, multiplex reverse-transcription loop-mediated isothermal amplification coupled with cascade invasive reaction using nanoparticles as a sensor; RT-PCR, reverse-transcription polymerase chain reaction.