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. 2017 Apr 4;12:2673–2687. doi: 10.2147/IJN.S131251

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© 2017 Zhou et al. This work is published and licensed by Dove Medical Press Limited

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New bone formation and mineralization measured histomorphometrically by fluorochrome-labeling analysis and Van Gieson’s picrofuchsin in rat calvarial defects implanted with HAP/chitosan and WH/chitosan. Tetracycline (yellow), alizarin red (red), and calcein (green) were intraperitoneally injected at week 2, week 4, and week 6, respectively. Column 4 represents merged images of the three fluorochromes of the same sample and column 5 represents Van Gieson’s picrofuchsin as a comparison.

Abbreviations: WH, whitlockite; HAP, hydroxyapatite.