TABLE 1.
Bacterial strains and plasmids used in this study
| Strains and plasmids | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Bacteria | ||
| Sinorhizobium meliloti | ||
| RCR2011 | SU47, wild type; Nod+ Fix+ | 33 |
| Rm1021 | Derivative of RCR2011; Smr | 23 |
| RmoxyR | Same as Rm1021 but with oxyR::uidA fusion (oxyR); Smr Nmr | This study |
| 1021-pRKAZ | Same as Rm1021 but with plasmid pRKAZ | This study |
| 1021-pdRKAZ | Same as Rm1021 but with plasmid pdRKAZ | This study |
| oxyR-pRKAZ | Same as RmoxyR but with plasmid pRKAZ | This study |
| oxyR-pdRKAZ | Same as RmoxyR but with plasmid pdRKAZ | This study |
| Escherichia coli | ||
| DH5α | F−supE44 ΔlacU169 (φ80dlacZΔM15) hsdR17(rK−mK+) recA1 endA1 gyrA96 thi-1 relA1 | Bethesda Research Laboratories |
| MT607 | pro-82 thi-1 hsdR17 supE44 recA56 | 8 |
| MT616 | MT607(pRK600) | 8 |
| Plasmids | ||
| pRK600 | ColE1 replicon with RK2 transfer region; Cmr | 8 |
| pBluescriptKS(+) | Derivative of pUC19 with f1(+)oriR; Apr | Stratagene |
| pCR-TOPO | Derivative of pUC19 with f1(+)ori; Apr Kmr | Invitrogen |
| pGEM-T | T vector with f1 oriR; Apr | Promega Corp. |
| pVO155 | Derivative of pUC119 | 29 |
| pKOK5 | Derivative of pSUP202, source of lacZ-Kmr; Apr Kmr | 19 |
| pBBR1-MCS-3 | Derivative of pBBR1-MCS; Tcr | 20 |
| pCR-TOPO-oxyR01 | pCR-TOPO vector with 271-bp PCR-amplified fragment of the oxyR gene | This study |
| pCR-TOPO-oxyR02 | pCR-TOPO vector with 1,780-bp PCR-amplified fragment with the oxyR gene and partial katA gene | This study |
| pGEMoxyR | pGEM-T, with 920-bp PCR-amplified fragment | This study |
| pVO-oxyR | pVO155, with BamHI-XbaI fragment of pCR-TOPO-oxyR01 | This study |
| pBBR-oxyR | pBBR IMCS-3, with SpeI-Apal fragment of pCR-TOPO-oxyR02 | This study |
| pBBR-doxyR | pBBR-oxyR, Sacl-Sacl fragment deleted | This study |
| pRKAZ | pBBR-oxyR, with fusion pkatA-lacZ | This study |
| pdRKAZ | pBBR-doxyR, with fusion pkatA-lacZ | This study |
| pBSKAI-1 | pBluescript, 1.4-kb EcoRI-ApaI fragment with partial katA | 12 |
a
Abbreviations: Tc, tetracycline; Sm, streptomycin; Ap, ampicillin; Km, kanamycin; Cm, chloramphenicol; Sp, spectinomycin.