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. 2005 Jan;187(1):296–303. doi: 10.1128/JB.187.1.296-303.2005

TABLE 5.

Synthesis yields of α-1,6 GOS, α-1,2 GOS, and total GOS in the presence of 200 g of sucrose liter−1 and 100 g of maltose liter−1a

Protein Oligosaccharide yield (%)
Total oligosaccharide yield (%) Sucrose consumption (%)
Yα-1,6 GOS Yα-1,2 GOS
L. mesenteroides NRRL B-1299 32.6 26.4 59 100
DSR-E 68.2 11.7 79.9 100
Δ(VZ) 59.2 21.4 80.6 100
Δ(CD2) 84.2 0.0 84.2 100
CD1 57.3 0.0 57.3 20
GBD-CD2 0.0 0.0 0.0 73
CD2 0.0 0.0 0.0 7
a

Reactions were carried out using the different truncated forms of DSR-E, with 0.5 U of crude cell extract ml−1, at 30°C in 20 mM sodium acetate buffer, pH 5.4.