TABLE 5.
Protein | Oligosaccharide yield (%)
|
Total oligosaccharide yield (%) | Sucrose consumption (%) | |
---|---|---|---|---|
Yα-1,6 GOS | Yα-1,2 GOS | |||
L. mesenteroides NRRL B-1299 | 32.6 | 26.4 | 59 | 100 |
DSR-E | 68.2 | 11.7 | 79.9 | 100 |
Δ(VZ) | 59.2 | 21.4 | 80.6 | 100 |
Δ(CD2) | 84.2 | 0.0 | 84.2 | 100 |
CD1 | 57.3 | 0.0 | 57.3 | 20 |
GBD-CD2 | 0.0 | 0.0 | 0.0 | 73 |
CD2 | 0.0 | 0.0 | 0.0 | 7 |
Reactions were carried out using the different truncated forms of DSR-E, with 0.5 U of crude cell extract ml−1, at 30°C in 20 mM sodium acetate buffer, pH 5.4.