TABLE 1.

Primers, oligonucleotides, and plasmids used in this study^a

Primer, oligonucleotide, or plasmid	Sequence or description	Use, source, or reference
Primers
Rv0980	5′-GTGGACACTAACTGTGGGGCCACGTT-3′; FP for Rv0980 upstream region	EMSA
mprAseq10	5′-GTTCTCGTCCGCGCGGGTTTCGCCTG-3′; RP for Rv0980 upstream region	EMSA
mprAseq10up	5′-ACCAACATTGGTGGAACGTGGTGC-3′; FP for mprA upstream region	EMSA, DNase I
mprAseq3	5′-CACGATCGTCGTCAACGACAAGAATT-3′; RP for mprA upstream region	EMSA, DNase I
mprBseq3up	5′-TCGGATGCCGATTCCGCAGCTT-3′; FP for pepD upstream region	EMSA, DNase I
Rv0983NotI	5′-GCGGCCGCCTAGGTTGCTCTTCCTGTACTAGG-3′; RP for pepD upstream region	EMSA, DNase I
Rm60F	5′-CCCGCATACCACGGCGCG-3′; FP for MprA box upstream of mprA	Competitive EMSA
Rm60R	5′-GGACACCAGTGTCGTCGCAGC-3′; RP for MprA box upstream of mprA	Competitive EMSA
mprAfpbs-F	5′-CCAATGTGGCTGATGTGGCTAAAC-3′; FP for mprA upstream region	DNase I
mprAfpbs-R	5′-AATCATGTCGAGCGCCTCA-3′; RP for mprA upstream region	DNase I
TOPOBamH1-fp	5′-GATCCACTAGTAACGGCCGC-3′; used to generate sequencing ladders	DNase I
mprAstart	5′-GGATCCGTGTCCGTGCGAATTCTTGTC-3′; mprA coding region	MprA expression
mprAstop	5′-GTCGACTCAGGGTGGTGTTTCACGTAG-3′; mprA coding region	MprA expression
mprARTsense	5′-CATTGCTGGAGATGCCTGATCG-3′	Real-time RT-PCR
mprARTantisense	5′-CTCGGTCTTGCGGCGTAG-3′	Real-time RT-PCR
pepDRTsense	5′-ACGACGGTAACCTTCTCTGAC-3′	Real-time RT-PCR
pepDRTantisense	5′-CGGAGACGCCCTGAACAC-3′	Real-time RT-PCR
sigARTsense	5′-GCCGATGACGACGAGGAG-3′	Real-time RT-PCR
sigARTantisense	5′-GGCGGATGCGGTGAGTTC-3′	Real-time RT-PCR
Oligonucleotides
1	5′-CACGGCGCGCCTCTCAGGCCAGTCTCAGGCGCTGCGACGACACTG-3′	EMSA
2	5′-CACGGCGCGCCAAAAAGGCCAGTCTCAGGCGCTGCGACGACACTG-3′	EMSA
3	5′-CACGGCGCGCCTCTCAGGCCAGAAAAAGGCGCTGCGACGACACTG-3′	EMSA
4	5′-CACGGCGCGCCAAAAAGGCCAGAAAAAGGCGCTGCGACGACACTG-3′	EMSA
5	5′-CACGGCGCGCCTCTCAGGCTCTCAGGCGCTGCGACGACACTG-3′	EMSA
6	5′-CACGGCGCGCCTCTCAGGCCTCTCAGGCGCTGCGACGACACTG-3′	EMSA
7	5′-CACGGCGCGCCTCTCAGGCCATCTCAGGCGCTGCGACGACACTG-3′	EMSA
8	5′-CACGGCGCGCCTCTCAGGCCAGCTCTCAGGCGCTGCGACGACACTG-3′	EMSA
9	5′-CACGGCGCGCCTCTCAGGCCAGCATCTCAGGCGCTGCGACGACACTG-3′	EMSA
10	5′-CACGGCGCGCCTCTCAGGCCAGCAGTCTCAGGCGCTGCGACGACACTG-3′	EMSA
11	5′-CACGGCGCGCCTCTCAGGCCAGCAGCTCTCAGGCGCTGCGACGACACTG-3′	EMSA
12	5′-CGGCGAACGTTATCTCAGTGGAATCTCAGTCCACGCGCGCAACCT-3′	EMSA
13	5′-CACGCGCGCAACCTAGTTGTGCAGTTACTGTTGAAAGCCACACCC-3′	EMSA
14	5′-CTCGGAGCACGGACATCGAGAACTCTCGGGGTTCGGCGAACGTTA-3′	EMSA
Plasmids
pTZ229	His-MprA expression plasmid; mprA coding sequence in pET15b	45
pTZ232	Trx-MprA expression plasmid; mprA coding sequence in pET32a	This study
pTZ324	His-MprA(D48A) expression plasmid; mprA(D48A) coding sequence in pET15b	45

^aFP, forward primer; RP, reverse primer; EMSA, electrophoretic mobility shift assay.