TABLE 2.
ORFs whose expression is up-regulated in cells adapted to 72°C compared to cells adapted to 95°C
| ORF | Description | TMDs | Mean (±SD) fluoresence intensity ratio
|
|
|---|---|---|---|---|
| 5 ha | Adapteda | |||
| PF0094 | Protein disulfide oxidoreductase (3) | 0 | 1.4 ± 0.7 (2.7) | |
| PF0101 | [Conserved hypothetical protein] | 0 | 2.5 ± 1.3 (5.5) | |
| PF0190 | [Conserved hypothetical protein] | 2 | 1.7 ± 0.7 (3.2) | 2.9 ± 0.6 (7.4) |
| PF0286 | [Conserved hypothetical protein] | 0 | 1.8 ± 0.6 (3.5) | |
| PF0296c | [Cobalamin biosynthesis protein] | 4 | 1.4 ± 0.8 (2.7) | |
| PF0297 | [Conserved hypothetical protein] | 0 | 1.9 ± 1.0 (3.6) | |
| PF0298 | [Conserved hypothetical protein] | 0 | 1.1 ± 0.4 (2.2) | |
| PF0318 | [Acylaminoacyl releasing enzyme] | 0 | 1.9 ± 0.7 (3.8) | 1.6 ± 0.6 (3.1) |
| PF0323 | [Conserved hypothetical protein] | 1 | 2.4 ± 1.3 (5.3) | |
| Unknown | ||||
| PF0324 | [Conserved hypothetical protein] | 1 | 4.6 ± 2.4 (23.8)b | 5.8 ± 2.1 (57.4) |
| PF0325 | [Conserved hypothetical protein] | 1 | 4.4 ± 1.1 (21.3) | 5.3 ± 2.1 (39.0) |
| PF0327 | [Conserved hypothetical protein] | 1 | 2.4 ± 1.9 (5.3) | |
| PF0371 | [Conserved hypothetical protein] | 12 | 1.4 ± 0.9 (2.6) | |
| PF0428 | [Alanyl-tRNA, C terminus] | 0 | 1.4 ± 0.7 (2.7) | |
| PF0429 | [Putative proline permease] | 13 | 3.9 ± 0.9 (14.6) | |
| PF0516 | [Conserved hypothetical protein] | 1 | 1.6 ± 0.6 (3.1) | |
| PF0665 | [Conserved hypothetical protein] | 4 | 1.6 ± 0.6 (2.9) | |
| PF0708 | [Conserved hypothetical protein] | 11 | 1.5 ± 0.3 (2.9) | |
| PF0709 | [Hypothetical protein] | 0 | 1.9 ± 0.7 (3.7) | |
| PF0718 | [Conserved hypothetical protein] | 5 | 2.0 ± 0.7 (3.9) | |
| PF0719 | [Hypothetical protein] | 0 | 2.5 ± 1.4 (5.5) | |
| PF0727 | [Hypothetical protein] | 0 | 2.1 ± 0.6 (4.4) | |
| PF0934 | [Conserved hypothetical protein] | 0 | 2.5 ± 0.5 (5.6) | 2.8 ± 0.8 (6.9) |
| Branched chain amino acid biosynthesis | ||||
| PF0935 | [Acetolactate synthase] | 0 | 2.5 ± 1.0 (5.6) | 3.3 ± 2.2 (9.9) |
| PF0936 | [Ketol-acid reductoisomerase] | 0 | 2.2 ± 1.4 (4.5) | |
| PF0943 | [Conserved hypothetical protein] | 0 | 2.0 ± 1.3 (3.9) | |
| PF1025 | [Conserved hypothetical protein] | 0 | 2.1 ± 0.5 (4.2) | |
| PF1062 | [Conserved hypothetical protein] | 0 | 2.6 ± 1.0 (6.2) | |
| Unknown | ||||
| PF1072 | [Conserved hypothetical protein] | 0 | 3.7 ± 0.6 (12.7) | |
| PF1073 | [Conserved hypothetical protein] | 4 | 3.3 ± 0.8 (9.8) | |
| PF1074 | [Conserved hypothetical protein] | 0 | 1.5 ± 0.6 (2.8) | 3.5 ± 0.8 (11.3) |
| Gyrase modulator | ||||
| PF1076 | [tldD protein homolog] | 0 | 2.0 ± 0.4 (4.1) | |
| PF1077 | [Protein interaction modulator] | 0 | 2.4 ± 0.5 (5.3) | |
| PF1100 | [Conserved hypothetical protein] | 1 | 1.8 ± 0.8 (3.5) | |
| PF1247 | [Conserved hypothetical protein] | 6 | 1.5 ± 0.4 (2.8) | |
| PF1270 | [Conserved hypothetical protein] | 0 | 1.9 ± 0.9 (3.8)b | 1.9 ± 0.7 (3.8) |
| Unknown | ||||
| PF1344 | [Maleate cis-trans isomerase] | 0 | 1.4 ± 0.4 (2.6) | 1.5 ± 0.4 (2.8) |
| PF1345 | [Arylsulfatase] | 0 | 1.6 ± 0.3 (3.0) | |
| PF1346 | [Pheromone shutdown protein] | 0 | 1.9 ± 0.6 (3.7) | |
| PF1347 | [Hypothetical protein] | 0 | 1.3 ± 0.6 (2.5) | |
| PF1408 | [Probable dipeptide-binding protein] | 1 | 1.7 ± 0.7 (3.2) | 2.2 ± 0.5 (4.7) |
| PF1418 | [Conserved hypothetical protein] | 0 | 1.6 ± 0.7 (2.9) | |
| PF1460 | [Conserved hypothetical protein] | 0 | 2.1 ± 1.2 (4.4) | |
| Vitamin biosynthesis | ||||
| PF1528 | [Imidazoleglycerol Pi synthase] | 0 | 2.4 ± 1.6 (5.2) | |
| PF1529 | [Ethylene-inducible protein homolog] | 0 | 1.8 ± 0.6 (3.5) | |
| PF1670 | [Alkaline serine protease] | 1 | 1.4 ± 0.9 (2.6) | |
| PF1677 | [Conserved hypothetical protein] | 6 | 2.8 ± 1.4 (6.8) | |
| Branched-chain amino acid biosynthesis | ||||
| PF1678 | [2-Isopropyl malate synthase] | 0 | 3.2 ± 1.1 (9.4) | |
| PF1679 | [3-Isopropyl malate dehydratase I] | 1 | 3.1 ± 1.4 (8.8) | |
| Aromatic amino acid biosynthesis | ||||
| PF1701 | [Chorismate mutase] | 0 | 3.9 ± 2.3 (14.5) | |
| PF1702 | [Aspartate aminotransferase] | 0 | 2.8 ± 0.9 (7.1) | |
| PF1703 | [Prephenate dehydrogenase] | 0 | 3.1 ± 0.8 (8.5) | |
| PF1711 | [Indole-3-glycerol phosphate synthase] | 0 | 1.6 ± 0.8 (3.0) | |
| PF1767 | KGOR, delta (47) | 0 | 1.8 ± 0.8 (3.4) | 1.5 ± 0.8 (2.8) |
| PF1944 | [Conserved hypothetical protein] | 0 | 1.9 ± 0.6 (3.8) | |
| Tungstoenzyme-4 | ||||
| PF1960 | [Aldehyde/aldose reductase] | 0 | 1.8 ± 0.8 (3.6) | |
| PF1961 | WOR4, W-oxidoreductase (44) | 0 | 1.9 ± 0.5 (3.8) | |
| PF1973 | [Conserved hypothetical protein] | 1 | 1.8 ± 0.7 (3.4) | 1.8 ± 0.7 (3.6) |
| PF2032 | [Conserved hypothetical protein] | 11 | 1.5 ± 0.7 (2.9) | |
a
See Table 1 and the text for details.
b
Statistics for the 5-h values: PF0324, P = 0.061; PF 1270, P = 0.017. For all other ORFs the P values are <0.010.
c
Potential operons are shown in bold where the intergenic distances are less than 16 nucleotides.