Figure 1.

Sperm capacitation and viability in the presence of peroxiredoxin (PRDXs) inhibitors. Human spermatozoa were capacitated in Biggers, Whitten and Whittingham medium (BWW) supplemented with 10% fetal cord serum ultrafiltrate (FCSu) with or without thiostreprton (TSP) or 1-Hexadecyl-3-(trifluoroethyl)-sn-glycero-2-phosphomethanol lithium (MJ33) for 3.5 h at 37°C. (A) Capacitation was determined by the induction of the acrosome reaction with lysophosphatidylcholine for 30 min at 37°C. *P < 0.05 versus all other groups using ANOVA and Bonferroni test (for capacitation), and *P < 0.05 for Kruskal–Wallis and Dwass–Steel–Chritchlow–Fligner test (for viability) for all pairwise comparisons. (B) Sperm viability was determined by the HOST test. Results are presented as mean ± SEM (n = 4 different donors). There were no significant differences among groups.