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Capacitation-associated actin polymerization is prevented by PRDXs inhibitors. Spermatozoa were capacitated in BWW supplemented with 10% FCSu with or without 10 μM TSP or MJ33 for 4 h at 37°C. Then, spermatozoa were labeled with phalloidin-FITC conjugate to determine actin polymerization as described in material and methods. Results are presented as mean ± SEM (n = 9 different donors). *P < 0.05 versus all other groups (ANOVA and Bonferroni test).
