Figure 6.

PRDXs inhibitors promote an oxidative stress that affects viability and increases lipid peroxidation in human spermatozoa. (A) Percoll-selected spermatozoa were incubated with 10% FCSu or without (control) in the presence or absence of different concentrations of TSP or MJ33 for 4 h at 37°C. Lipid peroxidation was determined by BODIPY C11 fluorescence by flow cytometry. Results are presented as mean ± SEM (n = 8 different donors). *P ≤ 0.01 FCSu-treated spermatozoa versus 10 μM TSP- and MJ33- or FeSo₄-treated sperm, **P ≤ 0.006 versus FCSu-treated spermatozoa. All comparisons were using Student's t-test with Bonferroni's correction. (B) Percoll-selected spermatozoa were capacitated with 10% FCSu in BWW medium in the absence or presence of TSP, MJ33 or PEN at 37°C for 4 h. Sperm viability was measured by the HOST test. Results are presented as the mean ± SEM (n = 4 different donors). Different letters denote significant differences (ANOVA and Bonferroni test, all P < 0.05).