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. 2017 Feb 28;61(4):468–480. doi: 10.1093/annweh/wxx014

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© The Author 2017. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — In vitro determination of coating stability using the alkaline comet assay. Pristine Q1 and Q1 coated with 0.5% hydrolysed PTMO (w/w quartz) were incubated for 0, 24, 48, 72, or 168 h in (A) AAF or (B) ALF and were subsequently washed three times and finally freeze-dried. Primary rat alveolar macrophages were then incubated for 4 h at 37°C with the different quartz samples or culture medium as negative (NC), Al₂O₃ as particulate negative (PNC), and DQ12 as quartz positive control, using a concentration of 75 μg cm⁻² and subjected to alkaline comet assay analyses. Data represent means ± SD of three independent experiments. */**/*** Significantly different from Al₂O₃: P ≤ 0.05/0.01/0.001, respectively, Student’s t-test for unpaired values, two tailed. °/°°Significantly different from uncoated Q1: P ≤ 0.05/0.01, respectively, Student’s t-test for paired values, two tailed.