Table 1.
In vivo determination of coating efficiency and stability. (A) Biochemical endpoints in BAL fluid 28 and 90 days post-treatment. (B) Cell counts in BAL fluid 28 and 90 days post-treatment.
| Treatment groups | Dose (mg rat−1) | (A) Biochemical endpoints in BAL fluid | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LDH (U l−1) | β-Glucuronidase (U l−1) | Total protein (mg l−1) | |||||||||||
| Day 28 | Day 90 | Day 28 | Day 90 | Day 28 | Day 90 | ||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||
| Physiological saline | 0.3 ml | 29 | 12.2 | 26 | 12.9 | 0.20 | 0.10 | 0.28 | 0.15 | 105 | 12.3 | 107 | 18.7 |
| — | — | — | — | — | — | — | — | — | — | — | — | ||
| Q1 | 1 | 82 | 19.3 | 108 | 33.6 | 0.64 | 0.28 | 1.06 | 0.36 | 194 | 22.0 | 329 | 63.3 |
| ** | — | n.s. | — | n.s. | — | n.s. | — | n.s. | — | ** | — | ||
| Q1 + 0.5% PTMO | 1 | 68 | 22.0 | 95 | 13.0 | 0.64 | 0.30 | 0.86 | 0.29 | 150 | 13.0 | 266 | 43.3 |
| n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | °° | * | n.s. | ||
| Q1 + 0.2% SIVO160 | 1 | 32 | 6.00 | 62 | 21.9 | 0.26 | 0.05 | 0.44 | 0.11 | 105 | 9.7 | 176 | 24.6 |
| n.s. | °°° | n.s. | ° | n.s. | ° | n.s. | °° | n.s. | °°° | n.s. | °°° | ||
| DQ12 | 1 | 148 | 37.7 | 286 | 117.2 | 2.08 | 1.33 | 5.28 | 2.67 | 291 | 60.4 | 409 | 74.2 |
| ** | — | ** | — | ** | — | ** | — | ** | — | ** | — | ||
| Treatment groups | Dose (mg rat−1) | (B) Cell counts in BAL fluid | |||||||||||
| Leukocytes (× 103 ml−1) | PMN (× 103 ml−1) | Lymphocytes (× 103 ml−1) | |||||||||||
| Day 28 | Day 90 | Day 28 | Day 90 | Day 28 | Day 90 | ||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||
| Physiological saline | 0.3 ml | 107 | 66.2 | 97 | 22.4 | 1 | 0.5 | 2 | 0.8 | 0 | 0.0 | 0.7 | 0.77 |
| — | — | — | — | — | — | — | — | — | — | — | — | ||
| Q1 | 1 | 261 | 129.1 | 235 | 150.8 | 85 | 54.2 | 99 | 97.5 | 0 | 0.0 | 11.6 | 8.23 |
| * | — | n.s. | — | ** | — | n.s. | — | — | — | * | — | ||
| Q1 + 0.5% PTMO | 1 | 140 | 24.5 | 221 | 106.0 | 18 | 11.2 | 72 | 34.1 | 0 | 0.0 | 7.9 | 5.75 |
| n.s. | n.s. | n.s. | n.s. | n.s. | °° | n.s. | n.s. | — | — | n.s. | n.s. | ||
| Q1 + 0.2% SIVO160 | 1 | 103 | 20.5 | 131 | 21.5 | 3 | 2.1 | 29 | 18.5 | 0 | 0.0 | 2.5 | 1.54 |
| n.s. | ° | n.s. | n.s. | n.s. | °°° | n.s. | n.s. | — | — | n.s. | °° | ||
| DQ12 | 1 | 353 | 120.2 | 651 | 324.0 | 105 | 48.1 | 249 | 111.0 | 0 | 0.0 | 11.4 | 8.45 |
| *** | — | ** | — | *** | — | ** | — | — | — | * | — | ||
Mean, mean value of five animals per group; —, not applicable; n.s., no statistically significant difference. */**/*** significantly different from saline-treated negative controls: P ≤ 0.05/0.01/0.001, respectively, Dunnett’s test (left row); °/°°/°°° significantly different from Q1-treated animals: P ≤ 0.05/0.01/0.001, respectively, Student’s t-test for unpaired values, two tailed (right row). In the Q1-treated group, one animal clearly did not react to quartz treatment with increased cell counts and was thus excluded from statistical analyses with regard to coating efficiency.