Fig 5. Flow cytometry analysis after five and eight weeks of hPBMC transplantation in HUMAMICE.

Flow cytometry analysis was done at five, eight weeks and three months after the hPBMC were transferred. Results of two representative mice (from nine analyzed mice) which were analyzed at five and eight weeks after transplantation are shown in Fig 5. (A) For the mouse which was analyzed at 5 weeks after transplantation, 11.6% of splenocytes were composed by hCD45⁺ cells, with 45.2% of this population being hCD19⁺, and 17.1% being hCD45⁺hCD3⁺ T lymphocytes: 29.0% of them were hCD3⁺CD8⁺ and 60.9% were hCD3⁺CD4⁺ T cells. (B) For the mouse which was analyzed at 8 weeks after transplantation, 9.7% of splenocytes were composed by hCD45⁺ cells, with 49.4% of this population being hCD19⁺, and 16.1% being T lymphocytes: 30.0% of them were hCD3⁺CD8⁺ and 59.0% were hCD3⁺CD4⁺ T lymphocytes.