Diazotrophic growth of the R. capsulatus parental strain, KS36, and selected mutant derivatives. To analyze diazotrophic growth via the alternative nitrogenase, R. capsulatus strains were grown in molybdenum-free minimal medium. KS36 (ΔnifHDK), TD6 (ΔnifHDK anfA), KS36-KS130A (ΔnifHDK anf1), KS36-NIRUB123 (ΔnifHDK ranR), and KS36-CS29 (ΔnifHDK ranT) are represented.