Skip to main content
. 2005 Jan;187(1):92–98. doi: 10.1128/JB.187.1.92-98.2005

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid Relevant characteristicsa Source or reference
E. coli
    DH5α Host for plasmid amplification 8
    S17-1 RP4-2 (Tc::Mu) (Km::Tn7) integrated in the chromosome 34
R. capsulatus
    B10S Spontaneous Smr mutant of R. capsulatus B10 16
    KS36 nifHDK deletion derivative of B10S; Spcr 35
    TD6 nifHDK deletion derivative of B10S; KmranfA::[Spcr] This study
S. cerevisiae CG1945 Host for R. capsulatus genomic library 7, 26
Plasmids
    pCS4 pGBT9.C derivative containing DBD-anf1 This study
    pCS28 pK18 derivative containing ranT::[Gmr] This study
    pCS29 pCS28 derivative containing Tcr-mob from pJQ18 This study
    pGAD424.C GAL4-AD; LEU2 Ampr 1
    pGAD-5 pGAD424.C derivative containing AD-ranT in-frame fusion This study
    pGAD-8 pGAD424.C derivative containing AD-ranT in-frame fusion This study
    pGAD-K16 pGAD424.C derivative containing AD-ranR in-frame fusion This study
    pGAD-P1 pGAD424.C derivative containing AD-ranR in-frame fusion This study
    pGBT9.C GAL4-DBD; TRP1 Ampr 1
    pKS130A Mobilizable pBR325 derivative containing anf1::[Gmr]; Ampr Cmr Tcr This study
    pKS131A pPHU236 derivative containing anfA-lacZ; Tcr 18
    pML5 Broad-host-range lacZ transcriptional fusion vector; Tcr 19
    pNIRUB96-II pSUP202 derivative containing [Kmr] from pBSL14 This study
    pNIRUB120 pBluescript KS derivative containing ranR This study
    pNIRUB121 pNIRUB120 derivative containing ranR::[Gmr] This study
    pNIRUB123 pNIRUB121 derivative containing Kmr-mob from pNIRUB96-II This study
    pNIRUB125 pSUP202 derivative containing ranT This study
    pNIRUB128 pML5 derivative containing ranR-lacZ This study
    pNIRUB132 pML5 derivative containing ranT-lacZ fusion This study
    pSA16 pSUP401 derivative containing anf1-lacZ; Tcr This study
    pTD7-2I pSUP401 derivative containing anfH-lacZ; Tcr 4
    pWKR202 Cmr Kmr Gmr 3
a

Amp, ampicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Spc, spectinomycin; Tc, tetracycline.