TABLE 1.
Strain or plasmid | Relevant characteristicsa | Source or reference |
---|---|---|
E. coli | ||
DH5α | Host for plasmid amplification | 8 |
S17-1 | RP4-2 (Tc::Mu) (Km::Tn7) integrated in the chromosome | 34 |
R. capsulatus | ||
B10S | Spontaneous Smr mutant of R. capsulatus B10 | 16 |
KS36 | nifHDK deletion derivative of B10S; Spcr | 35 |
TD6 | nifHDK deletion derivative of B10S; KmranfA::[Spcr] | This study |
S. cerevisiae CG1945 | Host for R. capsulatus genomic library | 7, 26 |
Plasmids | ||
pCS4 | pGBT9.C derivative containing DBD-anf1 | This study |
pCS28 | pK18 derivative containing ranT::[Gmr] | This study |
pCS29 | pCS28 derivative containing Tcr-mob from pJQ18 | This study |
pGAD424.C | GAL4-AD; LEU2 Ampr | 1 |
pGAD-5 | pGAD424.C derivative containing AD-ranT in-frame fusion | This study |
pGAD-8 | pGAD424.C derivative containing AD-ranT in-frame fusion | This study |
pGAD-K16 | pGAD424.C derivative containing AD-ranR in-frame fusion | This study |
pGAD-P1 | pGAD424.C derivative containing AD-ranR in-frame fusion | This study |
pGBT9.C | GAL4-DBD; TRP1 Ampr | 1 |
pKS130A | Mobilizable pBR325 derivative containing anf1::[Gmr]; Ampr Cmr Tcr | This study |
pKS131A | pPHU236 derivative containing anfA-lacZ; Tcr | 18 |
pML5 | Broad-host-range lacZ transcriptional fusion vector; Tcr | 19 |
pNIRUB96-II | pSUP202 derivative containing [Kmr] from pBSL14 | This study |
pNIRUB120 | pBluescript KS derivative containing ranR | This study |
pNIRUB121 | pNIRUB120 derivative containing ranR::[Gmr] | This study |
pNIRUB123 | pNIRUB121 derivative containing Kmr-mob from pNIRUB96-II | This study |
pNIRUB125 | pSUP202 derivative containing ranT | This study |
pNIRUB128 | pML5 derivative containing ranR-lacZ | This study |
pNIRUB132 | pML5 derivative containing ranT-lacZ fusion | This study |
pSA16 | pSUP401 derivative containing anf1-lacZ; Tcr | This study |
pTD7-2I | pSUP401 derivative containing anfH-lacZ; Tcr | 4 |
pWKR202 | Cmr Kmr Gmr | 3 |
Amp, ampicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Spc, spectinomycin; Tc, tetracycline.