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Cultured HPMEC were infected with H1N1 or vehicle (CTRL) for 1 hour, and then incubated for different periods. The expression of virus M gene in HPMEC was assessed by qPCR (30 cycles). The expression of M gene increases as culture time prolongs, indicating H1N1 infects cultured HPMEC effectively. *, p<0.05; ***, p<0.001. n = 6, from one of three repeated experiments.
