Skip to main content
. 2017 Apr 11;12(4):e0175188. doi: 10.1371/journal.pone.0175188

Table 1. Primer sequences for quantitative Real-time PCR.

Target gene Direction Sequence
TNFα Forward 5’TCAACCTCCTCTCTGCCATC3’
Reverse 5’CCAAAGTAGACCTGCCCAGA3’
IL-1β Forward 5’TGAAATGATGGCTTATTACAGTGG3’
Reverse 5’GTAGTGGTGGTCGGAGATTCGTAG3’
IL-6 Forward 5’CACACAGACAGCCACTCACC3’
Reverse 5’TTTTCTGCCAGTGCCTCTTT3’
CCL1 Forward 5’GGACACAGTTGGATGGGTTC3’
Reverse 5’GTAGTTTCGGGGACAGGTCA3’
CCL2 Forward 5’CTTCTGTGCCTGCTGCTCAT3’
Reverse 5’CGGAGTTTGGGTTTGCTTGTC3’
CXCL1 Forward 5’TTTTGAAATGTCAACCCCAAG3’
Reverse 5’GATCTCATTGGCCATTTGCT3’
CXCL8 Forward 5’TCAGAGACAGCAGAGCACAC3’
Reverse 5’GGCAAAACTGCACCTTCACA3’
Ifnα Forward 5’AAAGAAATGTAAGAAAGCTTTTGATGA3’
Reverse 5’TACACTTTGGCTCAGGACTCATTT3’
Ifnβ Forward 5’TGGGAGGCTTGAATACTGCCTCAA3’
Reverse 5’TCCTTGGCCTTCAGGTAATGCAGA3’
Icam1 Forward 5’CAATTTCTCATGCCGCACAG3’
Reverse 5’AGCTGGAAGATCGAAAGTCCG3’
Vcam1 Forward 5’TGAACCCAAACAGAGGCAGAGT3’
Reverse 5’GGTATCCCATCACTTGAGCAGG3’
E-selectin Forward 5’AGCAGAGTTTCACGTTGCAGG3’
Reverse 5’TGGCGCAGATAAGGCTTCA3’
Cd31 Forward 5’CAAACAGAAACCCGTGGAGATG3’
Reverse 5’ACCGTAATGGCTGTTGGCTTC3’
H1N1 M Forward 5’GGTGTCACTAAGCTATTCAA3’
Reverse 5’CAAAAGCAGCTTCTGTGGTC3’
HPRT Forward 5’GCAGACTTTGCTTTCCTTGG3’
Reverse 5’AAGCAGATGGCCACAGAACT3’