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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Cell. 2016 Nov 17;167(5):1252–1263.e10. doi: 10.1016/j.cell.2016.10.053

Figure 3. Photoabsorption by LITE-1 relies on its conformation.

Figure 3

(A) Denaturing LITE-1 with urea completely abolishes its photoabsorption. Shown are spectral data for mock- and urea-treated LITE-1. LITE-1 was treated with urea (4 M) for 5 min at room temperature prior to spectral analysis.

(B) Denaturing bacterial rhodopsin (bRho) with urea does not eliminate its photoabsorption. Urea treatment shifts bRho’s 568 nm absorbance peak to 370 nm. bRho was treated with urea (4 M) for 5 min at room temperature prior to spectral analysis.

(C) Denaturing LITE-1 with NaOH completely abolishes its photoabsorption. LITE-1 was treated with NaOH (0.1 M) for 5 min at room temperature prior to spectral analysis.

(D) Denaturing bacterial rhodopsin (bRho) with NaOH does not eliminate its photoabsorption. NaOH treatment shifts bRho’s 568 nm absorbance peak to 370 nm. bRho was treated with NaOH (0.1 M) for 5 min at room temperature prior to spectral analysis.

LITE-1 concentration: 0.4 μM. bRho concentration: 4 μM.

Also see Figure S4.