TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference(s), source, or source strain |
|---|---|---|
| E. coli GEc137 | E. coli DH1 fadR | 5 |
| E. coli DH10B | Cloning strain | Invitrogen |
| M. tuberculosis H37Rv | Virulent strain, possibly degrades alkanes | ATCC 27294 |
| P. fluorescens CHA0 | C10 to C28n-alkane-degrading biocontrol strain | 9 |
| P. fluorescens KOB2Δ1 | P. fluorescens CHA0 alkB knockout | 27 |
| P. putida GPo1 | C5 to C12n-alkane-degrading strain | 23, 33 |
| P. putida GPo12 | P. putida GPo1 cured of OCT | 14 |
| pCom8 | Expression vector, PalkB GmroriT alkS | 29 |
| pKKPalk | Expression vector, PalkB Apr | 29 |
| RK600 | Helper plasmid for triparental matings | 3 |
| pGEc47ΔB | ΔB derivative of pGEc47 | 34 |
| pCom8-alkB-GPo1 | pCom8 with alkB from P. putida GPo1 | 27 |
| pCom8-alkB-GPo1-H273A | pCom8-alkB-GPo1 with H273A mutation | This study |
| pCom8-alkB-GPo1-H273Q | pCom8-alkB-GPo1 with H273Q mutation | This study |
| pCom8-alkB-GPo1-W55S | pCom-alkB-GPo1 with W55S mutation | This study |
| pCom8-alkB-GPo1-W55C | pCom-alkB-GPo1 with W55C mutation | This study |
| pKKPalk-alkB-GPo1 | pKKPalk with alkB from P. putida GPo1 | This study |
| pKKPalk-alkB-GPo1-W55S | pKKPalk-alkB-GPo1 with W55S mutation | This study |
| pKKPalk-alkB-GPo1-W55C | pKKPalk-alkB-GPo1 with W55C mutation | This study |
| pCom8-alkB1-AP1 | pCom8 with alkB1 from A. borkumensis AP1 | 27, 32 |
| pCom8-alkB1-AP1-W58S | pCom8-alkB1-AP1 with W55S mutation | This study |
| pCom8-alkB1-AP1-W58C | pCom8-alkB1-AP1 with W55C mutation | This study |
| pCom8-alkB1-AP1-W58L | pCom8-alkB1-AP1 with W55L mutation | This study |
| pCom8-alkB1-AP1-W58G | pCom8-alkB1-AP1 with W55G mutation | This study |
| pCom8-alkB-H37Rv | pCom8 with alkB from M. tuberculosis H37Rv | 27 |
| pCom8-alkB-H37Rv-L69F | pCom8-alkB-H37Rv with L69F mutation | This study |
| pCom8-alkB-H37Rv-L69W | pCom8-alkB-H37Rv with L69W mutation | This study |