E. coli GEc137 |
E. coli DH1 fadR
|
5 |
E. coli DH10B |
Cloning strain |
Invitrogen |
M. tuberculosis H37Rv |
Virulent strain, possibly degrades alkanes |
ATCC 27294 |
P. fluorescens CHA0 |
C10 to C28n-alkane-degrading biocontrol strain |
9 |
P. fluorescens KOB2Δ1 |
P. fluorescens CHA0 alkB knockout |
27 |
P. putida GPo1 |
C5 to C12n-alkane-degrading strain |
23, 33
|
P. putida GPo12 |
P. putida GPo1 cured of OCT |
14 |
pCom8 |
Expression vector, PalkB GmroriT alkS
|
29 |
pKKPalk |
Expression vector, PalkB Apr
|
29 |
RK600 |
Helper plasmid for triparental matings |
3 |
pGEc47ΔB |
ΔB derivative of pGEc47 |
34 |
pCom8-alkB-GPo1 |
pCom8 with alkB from P. putida GPo1 |
27 |
pCom8-alkB-GPo1-H273A |
pCom8-alkB-GPo1 with H273A mutation |
This study |
pCom8-alkB-GPo1-H273Q |
pCom8-alkB-GPo1 with H273Q mutation |
This study |
pCom8-alkB-GPo1-W55S |
pCom-alkB-GPo1 with W55S mutation |
This study |
pCom8-alkB-GPo1-W55C |
pCom-alkB-GPo1 with W55C mutation |
This study |
pKKPalk-alkB-GPo1 |
pKKPalk with alkB from P. putida GPo1 |
This study |
pKKPalk-alkB-GPo1-W55S |
pKKPalk-alkB-GPo1 with W55S mutation |
This study |
pKKPalk-alkB-GPo1-W55C |
pKKPalk-alkB-GPo1 with W55C mutation |
This study |
pCom8-alkB1-AP1 |
pCom8 with alkB1 from A. borkumensis AP1 |
27, 32
|
pCom8-alkB1-AP1-W58S |
pCom8-alkB1-AP1 with W55S mutation |
This study |
pCom8-alkB1-AP1-W58C |
pCom8-alkB1-AP1 with W55C mutation |
This study |
pCom8-alkB1-AP1-W58L |
pCom8-alkB1-AP1 with W55L mutation |
This study |
pCom8-alkB1-AP1-W58G |
pCom8-alkB1-AP1 with W55G mutation |
This study |
pCom8-alkB-H37Rv |
pCom8 with alkB from M. tuberculosis H37Rv |
27 |
pCom8-alkB-H37Rv-L69F |
pCom8-alkB-H37Rv with L69F mutation |
This study |
pCom8-alkB-H37Rv-L69W |
pCom8-alkB-H37Rv with L69W mutation |
This study |