TABLE 2.

Growth rates of P. fluorescens CHA0, P. fluorescens KOB2Δ1, P. putida GPo1, P. putida GPo12(pGEc47ΔB), and derived recombinants on n-alkanes ranging from C₆ (hextane) to C₁₆ (hexadecane)

Strain or plasmidc	Growth rate (h−1) ona:
	C6	C8	C10c	C11c	C12	C13	C14	C16
GPo1	0.49	0.31	0.20	0.094	0.063	0.018	0	0
GPo12(pCom8-alkB-GPo1)	0.41	0.32	0.21	0.087	0.056	0.017	0	0
GPo12(pCom8-alkB-GPo1-W55S)	0.38	0.28	0.20	0.11	0.092	0.047	0	0
GPo12(pCom8-alkB-GPo1-W55C)	0.35	0.27	0.21	0.12	0.090	0.046	0	0
GPo12(pCom8-alkB1-AP1)		0.28	0.25	0.081	0.0087	0	0
CHA0b					0.019		0.019	0.053
KOB2Δ1(pCom8-alkB-GPo1)			0.009	0.013	0.0069	0.0020	0	0
KOB2Δ1(pCom8-alkB-GPo1-W55S)			0.0083	0.012	0.0068	0.0075	0.011	0.012
KOB2Δ1(pCom8-alkB-GPo1-W55C)			0.0087	0.012	0.0070	0.0077	0.012	0.013
KOB2Δ1(pCom8-alkB1-AP1)			0	0	0	0	0	0
KOB2Δ1*(pCom8-alkB1-AP1)			0.028	0.021	0.026	0.0091	0	0
KOB2Δ1*(pCom8-alkB1-AP1-W58S)			0.018	0.023	0.027	0.017	0.035	0.017
KOB2Δ1*(pCom8-alkB1-AP1-W58L)			0.014	0.024	0.022	0.0076	0.0051	0
KOB2Δ1(pCom8-alkB-H37Rv)			0.025	0.027	0.043	0.043	0.041	0.038
KOB2Δ1(pCom8-alkB-H37Rv-L69F)			0.024	0.0081	0	0	0	0
KOB2Δ1(pCom8-alkB-H37Rv-L69W)			0.011	0.0053	0	0	0	0

^aAverage growth rates of two parallel cultures inoculated from the same preculture (in all cases, the difference in growth rate was less than 10%). To reduce the toxicity of C₁₀ and C₁₁ to KOB2Δ1 recombinants, these alkanes were diluted in heptamethylnonane (1:3 for C₁₀ and 1:1 for C₁₁).

^bData are from reference 27.

^c*, uncharacterized mutation in KOB2Δ1 that allows functional expression of AP1 AlkB1.