Figure 2.

Purification and size fractionation of SAGA subunits. Gcn5-TAP (A), Ada3-TAP (B), Sgf29-TAP (C) and Spt20-TAP (D) were purified by a single affinity purification step from WT and not5Δ and purified proteins were separated on a 1–20% sucrose gradient. Proteins from the different fractions were TCA precipitated and loaded on SDS-PAGE, and transferred to a membrane that was probed with anti CBP, anti-Taf9 and anti-Spt3 antibodies as indicated. Fraction 1 is the lightest and fraction 12 the heaviest. The position of elution of SAGA and ADA are indicated.