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. 2016 Nov 29;45(3):1186–1199. doi: 10.1093/nar/gkw1059

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Model for the importance of the Not proteins and Tdh3 during co-translational assembly of SAGA. The ADA2, SPT20 and GCN5 mRNAs are tethered at their site of translation. The Ada2 protein is present at the site of Spt20 production. This needs expression of Not5. Not1 is present at the site where ADA2 is translated in a Not5-dependent manner. Not4 binds SPT20 mRNA if it can associate with Not1. Tdh3 associates with SPT20 mRNA if Not4 and Not5 are expressed. Tdh3 and Not5 are needed for integrity of Spt20. Appropriate nuclear localization of Gcn5, Ada2 and Spt20 needs Not5, and that of Gcn5 needs Tdh3. We propose that association of Not1 with ADA2 mRNA in polysomes needs Not5, and allows Not1 to promote association of Ada2, Not4 and Tdh3 with SPT20 mRNA in polysomes. This ensures appropriate association of Ada2 with Spt20 and integrity of Spt20, and finally interaction with newly produced Gcn5. In turn these co-translational events are the ones that ensure optimal integrity of SAGA. Otherwise the subunits are less faithfully incorporated into SAGA and will aggregate in the cytoplasm.