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. 2016 Nov 29;45(3):1255–1269. doi: 10.1093/nar/gkw1176

Figure 2.

Figure 2.

Stn1 dysfunction leads to subtelomere loss. (A) Southern hybridization probes (upper black boxes) and qPCR primer sets (lower open boxes) were designed for the subtelomeric region of chromosome II right arm (see Materials and Methods for sequences). The letter E refers to EcoRI sites. (B) Wild-type and stn1-1 were incubated at the restrictive temperature for the indicated times. EcoRI-digested genomic DNAs were analyzed by Southern hybridization. Each signal was first quantified and normalized to the signal from the control region; the values under the lanes represent the fold changes relative to the 25°C condition. (C) stn1-1 was cultured at 25°C and 36°C for 24 and 48 h. Fold changes of the abundance of qPCR products at 36°C compared to those at 25°C were calculated for each primer set. Error bars show mean values of three independent experiments with SD. The letter ρ represents the Pearson correlation between the Y-axis and X-axis from 0.4 to 30 kb.