Figure 6.

stn1 genetically interacts with rif1 and pmt3. (A) Ten-fold serial dilutions of the indicated strains were spotted onto SD plates (without camptothecin) and incubated for 5 days at 25°C, and 3 days at both 34°C and 36°C. (B) Cells were spotted onto SD (top) and SD + camptothecin plates (bottom) and incubated for 4 days at 25°C, and 3 days at 36°C, respectively. (C) stn1-1, stn1-1 + pmt3⁺, and stn1-1 rif1Δ were incubated at 25°C and shifted to 36°C for the indicated times. EcoRI-digested genomic DNAs were analyzed by Southern hybridization, using a telomere probe, and the same membrane was then stripped and re-hybridized with the control region probe. (D) stn1-1 rif1Δ cells were cultured at 25°C, 36°C for 24 h, and at 36°C for 48 h. qPCR and quantifications were carried out as for Figure 2C.