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. 2016 Nov 28;45(3):1233–1254. doi: 10.1093/nar/gkw1174

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Flip-out experiments combined with the PLA assays showing the interactions between HP1a and Mcm10, HP1a and RFC140, HP1a and dpolε255. Eye imaginal discs from flip-out experiments with UAS-Mcm10IR (A–C), UAS-HP1a_73-181IR (D–F), UAS-HP1a_119-206IR (G–I), UAS-RFC140IR (J–L) flies were performed PLA (red). (A–C) PLA between HP1a and Mcm10, (D–F) PLA between HP1a and RFC140, (G–I) PLA assays between HP1a and dpolε255. The cells expressing Mcm10 dsRNA (B), HP1a_73-181 dsRNA (E and H) and RFC140 dsRNA (K) are marked with GFP (green). The PLA signals were mostly detected in non-GFP clones (yellow arrows), while in GFP clones, the PLA signals were significantly reduced (white arrows). Scale bars indicate 20 or 30 μm. (a) indicates anterior, (p) indicates posterior.