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. 2016 Dec 20;45(3):1281–1296. doi: 10.1093/nar/gkw1214

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Induction of anemia drives more cells into the active stage and especially the G2/M phase of the cell cycle. A, B and C are different tSNE runwith the same parameters and the appearance of the detailed pattern varies (111) (A) The tSNE patterns of the whole population of HSC (purple) and MPP1 (green). Unlike MPP1, the majority of HSC cells are quiescent cells (middle cluster). (B) Comparative analysis of cells expressing high levels of S (red) and G2/M (purple) phase-specific genes in the combined population of HSC and MPP1. The colored cells (red and purple) are 10 cells each expressing highest levels of S or G2/M genes. (C) Both anemic HSC (blue dots) ls shift to the active stage, and the anemic MPP1 (red dots) shift towards the G2/M stage. (D) Three lineage expression levels in HSC/MPP1 cells from the control mice. Cells plotted according to increasing G2/M gene expression. The red, black and purple curves respectively refer to the expression per cell of erythroid precursor genes, of megakaryocyte genes, and of granulocyte/macrophage precursor genes. The curves were generated by loess smoothing of scatter plots. The gene expression levels are plotted on a log scale. (E) Three lineage levels of expression of genes in HSC/MPP1 cells from the anemic mice. Cells plotted as in Figure 5D. Note that a larger fraction of the cells from anemic mice were in the S/G2/M fraction.