Figure 2.

Telomere resolution is unaffected by ATP. (A) Schematic representation of the replicated telomere (rTel) substrate and the resultant hp telomere products of telomere resolution. The asterisks denote 5΄ ³²P-endlabels; S, substrate; hp1 and hp2, denote the hp telomere products; dots, the position of the scissile phosphates. The strand lengths in the rTel are from the scissile phosphates to the end of the DNA strand, the strand lengths in the hp telomeres indicate the total strand length of the products. (B) Representative 8% PAGE 1× TAE/0.1%SDS gel panel of a telomere resolution timecourse reaction performed in ATPase assay buffer at 37°C with 5.25 nM rTel and 74 nM ResT. The example shown is a reaction without ATP supplementation. (C) % Telomere resolution vs. time plots of telomere resolution assays performed without ATP addition, with ATP or with ATP-γ-S addition. Reactions were performed in triplicate and the mean and standard deviation are shown.