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Binding of Nab2 ZnF567 to GAL1 RNA. (A) Adding progressively larger amounts of ZnF567 depleted the amount of GAL1 RNA that migrated to the positive electrode in native agarose gels. (B) Similarly, in native PAGE gels the Nab2 protein was depleted by GAL1 RNA. At a 10:1 ratio most of the protein was depleted and there was substantial depletion even when it was added at a 40:1 ratio, consistent with each RNA binding multiple copies of the protein.
