TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli K-12 | ||
| CC118 | Δ(ara-leu) araD139 lacX74 galE galK phoA20 rpsE thi-1 argE rpoB recA | 23 |
| HW1273 | CC118 carrying pSS120::Tn1 (Apr Rifr) | 19 |
| ME2523 | HW1273 cpxA63::mini-Tn10 | This study |
| ME2526 | HW1273 cpxA22::mini-Tn10 | This study |
| ME2824 | HW1273 ΔcpxA | This study |
| ME2830 | HW1273 ΔcpxR | This study |
| S17-1 λ-pir | hsdR-M+thi pro recA RP4:2-Tc:Mu:Km:Tn7 λ-pir (Tpr Smr) | 10 |
| S. sonnei | ||
| HW383 | S. sonnei wild-type strain (Tcr) | 45 |
| MS390 | HW383 (Tcs) | This study |
| MS2824 | MS390 ΔcpxA | This study |
| MS2830 | MS390 ΔcpxR | This study |
| Plasmids | ||
| pBSL199 | Mini-Tn10 (Tcr) on R6K suicide vector | 2 |
| pEA225 | GST-InvE fusion on pGEX51 | This study |
| pGEMT-invE | PCR fragment amplified with invE4-invE5 primers on pGEMT-Easy vector | This study |
| pGEMT-virF | PCR fragment amplified with virF1-virF2 primers on pGEMT-Easy vector | This study |
| pHW848 | virF-lacZ fusion on pHSG595 (Cmr) | 27 |
| pSN0329 | E. coli cpxA on pACYC177 (Apr) | 27 |
| pJM0329 | pSN0329 Ω(bla::Tn7npt) (Kmr) | This study |
| pJM1717 | mxiC::Tn3-lac, ipaB-phoA fusion on pTH18kr (Kmr) | This study |
| pJM1718 | mxiC::Tn3-lac, ipaB-phoA fusion on pTH18cr (Cmr) | This study |
| pJM4320 | invE-lacZYA transcriptional fusion on pTH18rrnBT (Cmr) | This study |
| pJM4321 | invE-lacZYA translational fusion on pTH18rrnBT (Cmr) | This study |
| pTH18rrnBT | pTH18cs5 carrying PCR-amplified rrnBT1T2 sequence (Cmr) | This study |
| pTH18cs5 | pSC101 ori, single-copy plasmid (Cmr) | 16 |