Table 2. NTPase activities of Prp43 constructs.
| Proteins | NTP | kcat (s−1) | KM (μM) | kcat/KM (104.M−1.s−1) | R2 |
|---|---|---|---|---|---|
| Prp43-WT | ATP | 3.4 ± 0.2 | 55 ± 12 | 6.14 ± 0.44 | 0.88 |
| CTP | 15.4 ± 0.4 | 90 ± 8 | 16.74 ± 2.65 | 0.98 | |
| TTP | 12.8 ± 0.5 | 115 ± 15 | 11.25 ± 0.11 | 0.97 | |
| UTP | 9.7 ± 0.4 | 120 ± 15 | 8.15 ± 0.42 | 0.97 | |
| GTP | 3.7 ± 0.2 | 60 ± 10 | 6.33 ± 0.85 | 0.92 | |
| Prp43-WT + Pfa1 | ATP | 25.4 ±1.5 | 295 ± 45 | 8.67 ± 0.72 | 0.98 |
| CTP | 8.6 ± 1.1 | 445 ± 130 | 1.93 ± 0.14 | 0.94 | |
| Prp43–R159A | ATP | 7.3 ± 0.6 | 845 ± 175 | 0.85 ± 0.23 | 0.94 |
| CTP | 6.1 ± 0.3 | 935 ± 150 | 0.65 ± 0.13 | 0.95 | |
| Prp43–R159A + Pfa1 | ATP | No activity | |||
| CTP | No activity | ||||
| Prp43–F357A | ATP | 14.5 ± 0.6 | 90 ± 15 | 16.72 ± 0.31 | 0.95 |
| CTP | 12.7 ± 0.5 | 175 ± 20 | 7.32 ± 0.72 | 0.98 | |
| Prp43–F357A + Pfa1 | ATP | 6.9 ± 1.0 | 1385 ± 390 | 0.50 ± 0.26 | 0.88 |
| CTP | no activity |