Table 3. Affinity constants (Kd) of Prp43 (WT, R159A and F357A) for ssRNA in the presence of different NTPs.
| Nucleotide | Prp43 | Kd (nM) |
|---|---|---|
| No NTP | WT | 42 ± 17 |
| R159A | 20 ± 4 | |
| F357A | 14 ± 1 | |
| ATP | WT | 308 ± 55 |
| R159A | 18 ± 2 | |
| F357A | 87 ± 18 | |
| CTP | WT | 209 ± 42 |
| R159A | 8 ± 1 | |
| F357A | 43 ± 3 | |
| AMPPNP | WT | 8 ± 3 |
| R159A | 3 ± 0.8 | |
| F357A | 6 ± 1 | |
| ATP (10mM) | WT | 191 |
| ADP | WT | 144 |
| CDP | WT | 57 |
Binding affinities of Prp43-WT and the R159A and F357A mutants for a 21 nt ssRNA determined by filter-binding assays without or with different NTP at 1mM concentration unless otherwise stated (data in Supplementary Figure S2).