Figure 2.

Activity of SmYbeY on single-stranded RNA (ssRNA). In vitro reactivity of purified wild-type SmYbeY and the mutant variant SmYbeY-R69A on a 5΄-labeled 30mer ssRNA oligonucleotide (0.02 pmol/μl) whose sequence is indicated on bottom. Enzymes were used at a concentration of 5 μM in all assays. Buffering conditions and incubation times are indicated on top of the panels. Numbers to the left indicate sizes of the major reaction products. Reactions were analyzed on 7 M urea/20% polyacrylamide gels. C, control reactions.