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Site of insertion of Spinach/Broccoli sequences into 16S rRNA. (A) Secondary structure of 16S rRNA. The site of insertion in helix 33a (h33a) is boxed. The in vitro transcribed domain containing helices 33a and 33 is indicated by dashed lines. (B) Side view of 70S Escherichia coli ribosome (PDB code 2AW4 (50S) 2AVY (30S) (24)) with the tip of helix 33a highlighted. 16S and 23S rRNA molecules are shown in blue and red, respectively.
