Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 7;45(3):1404–1415. doi: 10.1093/nar/gkw794

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 8. — Degradation of Spinach-tagged 16S rRNA. (A) Total RNA from E. coli TA531 cells expressing Spinach-tagged ribosomes was separated on urea-PAGE and stained with DFHBI-1T (top) and SYBR Gold (bottom). 16S rRNA bands are indicated by arrows. (B) Ribosomes were purified from E. coli TA531 cells expressing Spinach-tagged 16S rRNA. RNA was separated on urea-PAGE and stained with DFHBI-1T (top) and SYBR Gold (bottom). (C) Fluorescent primer extension analysis of cleavage sites in Spinach-modified 16S rRNA. Fluorescent primer extension products generated using Cy5-labelled DNA primer were analysed on urea-PAGE. Regions corresponding to helix 33a, tRNA scaffold, Spinach and Baby Spinach are indicated by arrows.