FIG. 3.

Differentially expressed proteins in the D. nodosus fur mutant JIR3764. Shown are sections of representative gels of the wild-type strain VCS1703A and its fur mutant JIR3764. Spot numbers are designated according to the following key: F, up-regulated in fur mutant; W, down-regulated in fur mutant; L, identified in the cell lysate; and S, identified in the supernatant. Relevant bands are circled. Numbering for FS1 to FS5 is right to left, working down. Gels used for analytical purposes were loaded with 100, 45, and 30 μg of protein for cell lysate, total membrane, and secreted proteins, respectively. These values increased to 300, 100, and 200 μg, respectively, for successful micropreparative loadings.