TABLE 2.
Primer | Gene | Sequence (5′-3′)a | Use |
---|---|---|---|
16329 | fur | CGTAATGGTGTTTTTGTGCAAT | RT-PCR, PCR |
16441 | fur | AAGAAATTGGTTTGGCAACG | RT-PCR, PCR |
16661 | fur | GCTCTAGAAAGTCTGGCAGGAAATACGC | PCR |
16662 | fur | CGGAATTCGCCTTGCCGTTGTAAATGTAA | PCR |
16875 | fur | CATGCCCGCCATCATTGTGC | PCR |
16944 | fur | GACAGATGAAAGCGTTGACC | PCR |
18156 | fur | GCAATTTGAACAAGCGGGCAT | PCR |
18162 | fur | GGAATTCCCAATTTCTTCATCGGCAGCC | PCR |
18163 | fur | CCATCGATGAAAATTGCGACCACAAAAGCG | PCR |
18164 | fur | TCCCCCGCGGCGCGCAGTTCAACACGAAAT | PCR |
25946 | 16S rRNA | CGGAATGACTGGGCGTAAA | QRT-PCR |
25948 | 16S rRNA | CTAGGTTGAGCCCAGGGATTT | QRT-PCR |
25619 | sodA | GCAGCGCTATCCGCAATAAT | QRT-PCR |
25620 | sodA | CGCATCGCGTTGAAATAAAGA | QRT-PCR |
25606 | yfeA | ACAGAAGGCGCTTTTTCCTATTTA | QRT-PCR |
25621 | yfeA | CGGCCACACATAGGCTTCA | QRT-PCR |
25608 | cbiK | AATGGGAAATGAATAAAACTAGAAAGGA | QRT-PCR |
25604 | cbiK | GCATGGTTGAAATACCGCAAT | QRT-PCR |
25616 | speF | CGCTCCGGGTGATATGGTT | QRT-PCR |
25617 | speF | GCACCGTGGCAAACAGATTT | QRT-PCR |
Underlining represents sequence for cloning purposes.