TABLE 3.
Differentially expressed proteins in the D. nodosus fur mutant JIR3764 and their expression ratios as determined by QRT-PCR
| Spot no.a | Protein identification | Identity (%) | No. of peptides matched (% coverage)c | Relative gene expressiond |
|---|---|---|---|---|
| FL1 | YfeA, iron transport protein | 64 | 10 (46) | 1.44 ± 0.35 |
| FS1 | YfeA, iron transport protein | 64 | 11 (60) | 1.44 ± 0.35 |
| FS2 | YfeA, iron transport protein | 64 | 11 (48) | 1.44 ± 0.35 |
| FS4 | CbiK domain, ABC-type cobalt transport | 29 | 15 (67) | 4.28 ± 0.97 |
| FS5 | CbiK domain, ABC-type cobalt transport | 29 | 19 (61) | 4.28 ± 0.97 |
| FS6 | CbiK domain, ABC-type cobalt transport | 29 | 11 (45) | 4.28 ± 0.97 |
| WL1 | Ornithine decarboxylaseb | 64 | 16 (23) | 0.38 ± 0.24 |
| WS1 | SodA, manganese superoxide dismutase | 58 | 9 (48) | 0.35 ± 0.02 |
a
F, protein up regulated in fur mutant; W, protein present in wild-type, down-regulated in fur mutant; L, present in cell lysate; and S, present in secreted sample.
b
Spot corresponded to a truncated form of a larger ORF.
c
Number of experimentally derived mass peaks that match to the in silico derived peaks for that protein (degree of coverage of proteins conferred by the residues represented by matching peaks).
d
Expressed as the ratio of the specific gene expression level in the fur mutant compared to wild-type normalized to the level of expression of the 16S rRNA gene.