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. 2016 Sep 12;45(3):1442–1454. doi: 10.1093/nar/gkw816

Figure 3.

Figure 3.

S411A enzymatic and signaling activities differ from WT RIG-I. (A) Steady state ATP hydrolysis by wild type and S411A RIG-I proteins stimulated with varying concentrations of the RNA hairpin 5΄ppp10L. (B) IFN-β induction in HEK 293T cells transfected with the indicated amount (ng) of the constitutive expression plasmid pUNO-hRIG-I containing either wild type or mutant RIG-I constructs. Cells expressing the indicated construct were challenged by transfection of 5΄ppp10L. (C) IFN-β induction of WT RIG-I and S411A in HEK-293T cells challenged with 5΄OH10L, 5΄ppp10L and mock. (D) IFN-β induction in HEK 293T cells transfected with the indicated amount (ng) of the constitutive expression plasmid pUNO-hRIG-I containing either wild type or mutant RIG-I constructs. Cells expressing the indicated construct were challenged by transfection of OH10L.